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PROTOCOL FOR SINGLE CELL mRNA AMPLIFICATION (REVERSE NORTHERN ANALYSIS)
source: dartmouth.edu
I. First round amplification
A.First strand cDNA synthesis
1.Add to single cell:
Cell contents plus recording solution 3ul
1x RT buffer 17.5ul
dGTP (10mM) 1ul
dATP (10mM) 1ul
dTTP (10mM) 1ul
dCTP (10mM) 1ul
Oligo-dT-T7(100ng/ul) 1ul
DTT(100mM) 3ul
RNasin 0.5ul
AMV-RT(25U/ul) 1ul