Phosphate buffered saline (abbreviated as PBS) is a buffer solution commonly used in biological research. It is a salty solution containing sodium chloride, sodium phosphate, and (in some formulations) potassium chloride and potassium phosphate.
Phosphate buffered saline (abbreviated as PBS) is a buffer solution commonly used in biological research. It is a salty solution containing sodium chloride, sodium phosphate, and (in some formulations) potassium chloride and potassium phosphate.
Last Updated ( Thursday, 23 June 2011 10:11 )
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Mary-Ann Campbell's method.
This is a quick method of getting paraformaldehyde (PFM) into solution. It is a good alternative to the traditional methods which can take hours, and has the advantage that it is so quick
Do not autoclave solutions containing ethanol, isopropanol or MOPS; use sterile filtration if necessary.
Buffer AE (elution buffer for genomic DNA preps)
Classification:
Fixatives may be classed as precipitant (P) or non-precipitant (NP) according to their effect on tissue protein.
Primary fixatives are: