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RAPD PCR
RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers ...
avery.rutgers.edu/WSSP/StudentScholars/project/archives/onions/rapd.html
RAPD PCR Reaction Mixes:
Type, Initialize, X, Denature, Anneal, Extend, Termination. RAPD 10-mer, 95C, 5min, 45, 94C, 0:30, 35C, 1:00, 72C, 2:00, 72C, 5:00. PCR 18+mer, 95C, 5:00 ...
wheat.pw.usda.gov/~lazo/methods/lazo/pcrmeth0.html
Handbook for DNA isolation, RAPD-PCR and PCR-RFLP
A standard RAPD-PCR reaction is performed in volumes of 25.0 m l containing 50 mM KCl, 10 mM Tris-HCl pH 9.0, 1.5 mM MgCl2, 0.001% gelatine, ...
www.toyen.uio.no/botanisk/brochmann/handbook.htm