E-BioTek: Biomedical Protocols for Molecular, Cellular & Experimental Life Sciences
Unlock the knowledge behind every successful experiment
Welcome to E-BioTek.com, a premier platform dedicated to life science researchers, laboratory professionals, and students in the fields of molecular biology, cell biology, biochemistry, immunology, and biomedical innovation. We provide access to a vast, organized library of validated laboratory protocols, analytical techniques, and experimental guides to support high-quality, reproducible research.
Whether you are performing DNA extraction, designing a gene knockout experiment, analyzing protein interactions, culturing stem cells, or isolating metabolites using GC/MS or LC-MS, E-BioTek.com offers step-by-step methods, practical tips, and access to essential scientific tools.
About us
E-BioTek.com was founded with a vision: to create a free and centralized platform where researchers can access and contribute high-quality protocols that have been proven in real laboratories. In today’s fast-paced scientific environment, reproducibility, efficiency, and accessibility are key to experimental success.
Our Mission
To empower the life sciences community by offering open access to high-quality, reliable, and comprehensive laboratory protocols.
Molecular Biology Protocols
Core techniques for DNA, RNA, protein analysis, and gene manipulation
- DNA and RNA extraction
- Combined DNA/RNA/protein isolation
- PCR, RT-PCR, and qPCR
- Probe labeling and hybridization
- Mutation detection (SSCP)
- Loss of heterozygosity (LOH)
- Southern and Northern blot
- In situ hybridization (RNA–RNA)
- siRNA and CRISPR knockout
- Chromosome pattern analysis
- Subcellular fractionation
- Western blot and EMSA
- 2D gel electrophoresis
- Flow cytometry (FCM)
- ELISA
- Immunoprecipitation
- Thin-layer chromatography (TLC)
- Gene transfection and Tet-On/Tet-Off expression systems
- Reporter and promoter analysis
Cell Biology Protocols
Simple and effective methods for culturing and analyzing cells.
- General cell culture (adherent, suspension, primary)
- Cancer cell line maintenance
- Isolation of fibroblasts, endothelial cells, and white blood cells
- Co-culture and transwell systems
- Cell counting and viability assays
- MTT and BrdU cell proliferation tests
- Cell cycle and apoptosis analysis (FACS, Annexin V, TUNEL)
- Migration and invasion assays
- Aorta ring assay for angiogenesis
- Transfection with GFP or luciferase
- Live-cell imaging and GFP tracking
- Brain microvessel endothelial cell isolation
- Colony formation and clonogenic assays
- Stem cell culture and differentiation
Animal Experiments
- Cancer xenograft models
- Tumor monitoring and tissue sampling
- Experimental ethics and animal handling
Metabolism & GC/MS
Biochemical profiling and metabolic analysis
- GC/MS and LC/MS background
- Glucose and ribose metabolism
- Deoxyribose and lactate assays
- Fatty acid and amino acid analysis
- Urea, cholesterol, and bile acids
- Choline incorporation protocols
- Isotope Ratio Mass Spectrometry (IRMS)
- Sample preparation for MS-based analysis
Immunology & Histology
Visualize, label, and explore immune and structural biology
- Slide preparation (silanized, charged)
- H&E staining for tissue analysis
- Immunoperoxidase and immunofluorescence staining
- Confocal microscopy and laser scanning
- Antibody purification
- Laser Capture Microdissection (LCM)
- Immunoprecipitation protocols
HPLC Applications
Use chromatography to separate, detect, and identify biomolecules
- Extraction of bioactive plant compounds
- HPLC for protein, lipid, and secondary metabolites
- Fractionation and compound isolation
Special Topics
Advanced, interdisciplinary, and trending protocols
- Apoptosis detection methods
- Special stains for cells and subcellular fractions
- Microarray and gene expression profiling
- Gene therapy methods for cancer
- DNA/RNA/protein extraction from tissue cultures
- Gene transfection techniques
- Conditional expression systems (Tet-On/Tet-Off)
- Stem cell research protocols
- Active compound purification from natural sources
