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Coat the Plate:
1. Dilute Low-Endotoxin/Azide-Free sterile unlabeled capture antibody (BioLegend’s
LEAF™ format antibodies are specifically designed for this assay) to a
final concentration of 0.5–4 μg/ml in sterile Coating Buffer and transfer 100
μl/well to a high affinity binding PVDF membrane ELISPOT plate (e.g., Millipore;
Cat. No. MAIPS-4510).
2. Store plates overnight in humidified box at 4°C or at 37°C for ≥ 4 hours in
humidified atmosphere.