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HOW TO ... Clone with the promega PGEM-T kit (by Rutgers, November 2004)
Media and plates
1.1- Equipment
§ gas burner
§ rotating plate
§ aluminium rack for PCR tubes
1.2- Reagents
§ Tryptone: Fisher BP1421-500
§ yeast extract: Fisher BP1422-500
§ NaCl: Sigma S-3014
§ MgCl2: ????
§ glucose: ???
§ KCl: ???
§ Agar: DIFCO 214010
§ DMSO: ????